Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos

Brian S. Santos, Maria Rowena R. Romana-Eguia, Zubaida U. Basiao, Minoru Ikeda

研究成果: Letter査読

5 被引用数 (Scopus)

抄録

The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.

本文言語English
ページ(範囲)451-453
ページ数3
ジャーナルConservation Genetics Resources
7
2
DOI
出版ステータスPublished - 2015 6 1

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 遺伝学

フィンガープリント

「Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル