Development and application of capillary electrochemical sensors

Masao Sugawara, Ayumi Hirano

研究成果: Article査読

抄録

The present paper describes analytical aspects of electrochemical ultramicrosensors constructed using glass capillaries having a tip diameter of 0.5-12 μm. The constructed sensors included open capillary electrodes with the sampling ability of analytes based on electrokinetic phenomena and capillarity, membrane sensors in combination with planar bilayer lipid membranes (BLMs) and excised biomembrane sensors. The open capillary sensor had abilities of electrokinetic sampling and charge-selective detection of analyte ions. Selective detection of dopamine in a neutral solution after being transported into an inner acidic solution of the open capillary sensor was also demonstrated. The constructed capillary membrane sensors include (i) a novel sensor that mimics the mode of signal transduction displayed by G protein-linked receptors with BLMs containing receptors and a single gramicidin channel, (ii) an excised biomembrane sensor that allows the detection of arachidonic acid selectively over its metabolites and other fatty acids, and (iii) L-glutamate sensors using BLMs and biomembranes containing a glutamate receptor. The response characteristics of these capillary sensors were studied in terms of the working principle, dynamic range, sensitivity and selectivity. The application of these sensors to detection of signaling molecules in mouse brain slices was also demonstrated.

本文言語English
ページ(範囲)1121-1133
ページ数13
ジャーナルBUNSEKI KAGAKU
51
12
DOI
出版ステータスPublished - 2002 12
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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