Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique: Sex difference in healthy volunteers and patients with diabetes mellitus

Naoto Suzuki, Takanori Hishinuma, Toshihide Saga, Jo Sato, Takayoshi Toyota, Junichi Goto, Michinao Mizugaki

研究成果: Article査読

32 被引用数 (Scopus)

抄録

We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.

本文言語English
ページ(範囲)383-389
ページ数7
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
783
2
DOI
出版ステータスPublished - 2003 1 15

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

フィンガープリント 「Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique: Sex difference in healthy volunteers and patients with diabetes mellitus」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル