Determination of sphingolipids by LC-MS/MS

Tomohiro Takahashi, Chihiro Takeda, Kohei Saito, Naoto Suzuki, Hiroki Tsukamoto, Yoshihisa Tomioka

研究成果: Chapter

抄録

To investigate the biological functions and roles of sphingolipids, sensitive and compound-specific methods are required to measure their levels in biological samples. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization (ESI) is suitable for the reliable simultaneous analysis of multiple compounds. In addition, the selected reaction monitoring (SRM) mode of tandem mass spectrometry (MS/MS) is effective to quantify with high sensitivity and selectivity. Therefore, LC-MS/MS came to be utilized for simultaneous analysis of the sphingolipids in vivo. Useful methods for the sphingolipids and related features are also summarized. The following protocol demonstrates information on determination of sphingolipids, especially sphingosine and sphingosine-1- phosphate, by LC-ESI-MS/MS in biological samples such as cell lysates, plasma, serum, orurine.

本文言語English
ホスト出版物のタイトルBioactive Lipid Mediators
ホスト出版物のサブタイトルCurrent Reviews and Protocols
出版社Springer Japan
ページ357-370
ページ数14
ISBN(電子版)9784431556695
ISBN(印刷版)9784431556688
DOI
出版ステータスPublished - 2015 1 1

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 医学(全般)

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