Determination of lipid hydroperoxides in low density lipoprotein from human plasma using high performance liquid chromatography with chemiluminescence detection

Teruo Miyazawa, Kenshiro Fujimoto, Shin‐Ichi ‐I Oikawa

研究成果: Article査読

54 被引用数 (Scopus)

抄録

A high performance liquid chromatographic system with chemiluminescence detection (HPLC‐CL) was used for determining phospholipid hydroperoxides in human plasma low density lipoprotein (LDL). This system involved separation of phospholipids from LDL‐total lipids with normal phase silica gel HPLC and post‐column detection of hydroperoxide‐dependent chemiluminescence produced by luminol oxidation during the reaction of hydroperoxide with cytochrome c‐haeme. By using HPLC‐CL, we could detect phosphatidylcholine hydroperoxide (PCOOH) in human plasma LDL, and the LDL‐PCOOH concentration was significantly higher in patients with atherosclerosis and hyperlipidemia than that of healthy volunteers. The LDL‐PCOOH level was proportional to the plasma total cholesterol concentration.

本文言語English
ページ(範囲)131-134
ページ数4
ジャーナルBiomedical Chromatography
4
3
DOI
出版ステータスPublished - 1990 1 1

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

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