Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura; Kiyotaka Nakagawa; Yuko Saito; Kenji Yamagishi; Masahiro Suzuki; Kohji Yamaki; Hiroshi Shinmoto; Teruo Miyazawa

doi:10.1021/jf0306901

Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

未来科学技術共同研究センター

研究成果: Article › 査読

85 被引用数 (Scopus)

抄録

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

本文言語	English
ページ（範囲）	1415-1418
ページ数	4
ジャーナル	Journal of Agricultural and Food Chemistry
巻	52
号	6
DOI	https://doi.org/10.1021/jf0306901
出版ステータス	Published - 2004 3月 24

ASJC Scopus subject areas

化学 (全般)
農業および生物科学（全般）

文献へのアクセス

10.1021/jf0306901

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引用スタイル

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abstract = "A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.",

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AU - Kimura, Toshiyuki

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AU - Saito, Yuko

AU - Yamagishi, Kenji

AU - Suzuki, Masahiro

AU - Yamaki, Kohji

AU - Shinmoto, Hiroshi

AU - Miyazawa, Teruo

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