Detection of serum antibody against bovine coccidiosis by using eimeria tenella antigen

Tatsuya Uchida, Hasbullah, Yutaka Nakai, Keiji Ogimoto

研究成果: Article査読

抄録

Antigens of Eimeria tenella sporulated oocysts were applied to detect serum antibody of calf infected with Eimeria. Polypeptides of E. tenella oocyst were strongly proved with the infected bovine serum in immunoblotting. The infected serum also showed higher absorbances than that of normal serum in enzyme linked immunosorbent assay (ELISA) using E. tenella oocyst antigen. Suitable conditions for the ELISA were obtained by using soluble antigen of E. tenella oocysts at 2.5μg/ml and bovine serum at 200 fold dilution.

本文言語English
ページ(範囲)169-171
ページ数3
ジャーナルJournal of Veterinary Medical Science
57
1
DOI
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 獣医学(全般)

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