Detection of protein-protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry

Seigo Kodera, Toshihide Okajima, Hidehiko Iwabuki, Daisuke Kitaguchi, Shun'ichi Kuroda, Tatsuo Yoshinobu, Katsuyuki Tanizawa, Masamitsu Futai, Hiroshi Iwasaki

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO2/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100nm) was correlated with the thickness of the protein layer immobilized on SiO 2/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO 2/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future.

本文言語English
ページ(範囲)65-70
ページ数6
ジャーナルAnalytical Biochemistry
321
1
DOI
出版ステータスPublished - 2003 10月 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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