TY - JOUR
T1 - Detection of Picomole Levels in Lipid Hydroperoxides by a Chemiluminescence Assay
AU - Miyazawa, Teruo
AU - Fujimoto, Kenshiro
PY - 1987/1/1
Y1 - 1987/1/1
N2 - A highly sensitive and simple chemiluminescent method for the quantitation of lipid hydroperoxides at the picomole level is described. The method is based on detecting the chemiluminescence generated during the oxidation of luminol by the reaction with hydroperoxide and cytochrome c under mild conditions. A semilogarithmic relationship was observed between the hydroperoxide added and the chemiluminescence produced. For lipid hydroperoxides, cytochrome c was a most favorable catalyst for generating the chemiluminescence, rather than cytochrome c heme peptide and horseradish peroxidase. This method had high sensitivity to methyl linoleate hydroperoxide, arachidonic acid hydroperoxide and cholesterol hydroperoxide, but low to t-butyl hydroperoxide, t-butyl perbenzoate, diacyl peroxides (lauroyl peroxode and benzoyl peroxide) and dialkyl peroxides (di-t-butyl peroxide and dicumyl peroxide).
AB - A highly sensitive and simple chemiluminescent method for the quantitation of lipid hydroperoxides at the picomole level is described. The method is based on detecting the chemiluminescence generated during the oxidation of luminol by the reaction with hydroperoxide and cytochrome c under mild conditions. A semilogarithmic relationship was observed between the hydroperoxide added and the chemiluminescence produced. For lipid hydroperoxides, cytochrome c was a most favorable catalyst for generating the chemiluminescence, rather than cytochrome c heme peptide and horseradish peroxidase. This method had high sensitivity to methyl linoleate hydroperoxide, arachidonic acid hydroperoxide and cholesterol hydroperoxide, but low to t-butyl hydroperoxide, t-butyl perbenzoate, diacyl peroxides (lauroyl peroxode and benzoyl peroxide) and dialkyl peroxides (di-t-butyl peroxide and dicumyl peroxide).
UR - http://www.scopus.com/inward/record.url?scp=85004561012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004561012&partnerID=8YFLogxK
U2 - 10.1271/bbb1961.51.2569
DO - 10.1271/bbb1961.51.2569
M3 - Article
AN - SCOPUS:85004561012
VL - 51
SP - 2569
EP - 2573
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 9
ER -