Detection of Picomole Levels in Lipid Hydroperoxides by a Chemiluminescence Assay

Teruo Miyazawa, Kenshiro Fujimoto

研究成果: Article査読

34 被引用数 (Scopus)

抄録

A highly sensitive and simple chemiluminescent method for the quantitation of lipid hydroperoxides at the picomole level is described. The method is based on detecting the chemiluminescence generated during the oxidation of luminol by the reaction with hydroperoxide and cytochrome c under mild conditions. A semilogarithmic relationship was observed between the hydroperoxide added and the chemiluminescence produced. For lipid hydroperoxides, cytochrome c was a most favorable catalyst for generating the chemiluminescence, rather than cytochrome c heme peptide and horseradish peroxidase. This method had high sensitivity to methyl linoleate hydroperoxide, arachidonic acid hydroperoxide and cholesterol hydroperoxide, but low to t-butyl hydroperoxide, t-butyl perbenzoate, diacyl peroxides (lauroyl peroxode and benzoyl peroxide) and dialkyl peroxides (di-t-butyl peroxide and dicumyl peroxide).

本文言語English
ページ(範囲)2569-2573
ページ数5
ジャーナルAgricultural and Biological Chemistry
51
9
DOI
出版ステータスPublished - 1987 1 1

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

フィンガープリント

「Detection of Picomole Levels in Lipid Hydroperoxides by a Chemiluminescence Assay」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル