Detection of Novel Red-Colored, Ultra-Weak Chemiluminescence from Carcinogens

Kazumi Osada, Yuji Furukawa, Michio Komai, Shuichi Kimura, Kohya Hishinuma, Mika Kimura, Humio Inaba

研究成果: Article査読

2 被引用数 (Scopus)

抄録

We previously reported that benzo-(a)-pyrene (BP) and methylcholanthrene (MC) emitted blue-colored ultra-weak chemiluminescence (CL) during the metabolism in a post mitochondrial fraction system. In this present study, we used a single-photon counting system (SPC) equipped with a photomultiplier having a spectral response in the red-color region to detect the CL from BP, MC, and 3-nitrofluoranthene (3-NF). As a result, we discovered that a novel red-region CL was emitted immediately after initiation of the metabolic reaction in this system. In the case of BP and MC, each had a double phase CL emission; that is, they initially emitted red CL, which was detected by the SPC, and thereafter, blue CL, which was detected with an OX-71 chemiluminescence analyzer. However, in the case of 3-NF, although strong red CL emission was recorded, no blue-colored CL appeared to be emitted. The emissions of red CL were increased in systems in which D20 was added (final concentration of 66%), and decreased by the addition of β-carotene or α-tocopherol. This result suggests that the main cause of CL was singlet oxygen.

本文言語English
ページ(範囲)1-6
ページ数6
ジャーナルJournal of Clinical Biochemistry and Nutrition
14
1
DOI
出版ステータスPublished - 1993

ASJC Scopus subject areas

  • 医学(その他)
  • 栄養および糖尿病
  • 臨床生化学

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