Detection of immunoreactive endothelin in plasma of hemodialysis patients

Kazuhito Totsune, Toraichi Mouri, Kazuhiro Takahashi, Minoru Ohneda, Masahiko Sone, Takao Saito, Kaoru Yoshinaga

研究成果: Article査読

56 被引用数 (Scopus)

抄録

Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C18 cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C18 cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

本文言語English
ページ(範囲)239-242
ページ数4
ジャーナルFEBS Letters
249
2
DOI
出版ステータスPublished - 1989 6 5

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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