Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5

Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Mika K. Kaneko, Yukinari Kato

研究成果: Article査読

30 被引用数 (Scopus)

抄録

CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (CD44s), which lacks variant exons, is widely expressed on the surface of most tissues and all hematopoietic cells. In contrast, CD44 variant isoforms show tissue-specific expression patterns and have been extensively studied as both prognostic markers and therapeutic targets in cancer and other diseases. In this study, we immunized mice with CHO-K1 cell lines overexpressing CD44v3-10 to obtain novel anti-CD44 mAbs. One of the clones, C44Mab-5 (IgG1, kappa), recognized both CD44s and CD44v3-10. C44Mab-5 also reacted with oral cancer cells such as Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4 using flow cytometry. Moreover, immunohistochemical analysis revealed that C44Mab-5 detected 166/182 (91.2%) of oral cancers. These results suggest that the C44Mab-5 antibody may be useful for investigating the expression and function of CD44 in various cancers.

本文言語English
ページ(範囲)64-68
ページ数5
ジャーナルBiochemistry and Biophysics Reports
14
DOI
出版ステータスPublished - 2018 7

ASJC Scopus subject areas

  • 生物理学
  • 生化学

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