Detection of EGFR gene mutation by mutation-oriented LAMP method

Naoyuki Matsumoto, Akira Kumasaka, Tomohiro Ando, Kazuo Komiyama

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Background/Aim: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for non-small cell lung cancer. EGFR gene mutations at codons 746-753 promote constitutive EGFR activation and result in worst prognosis. However, these mutations augment the therapeutic effect of EGFR-tyrosine kinase inhibitor. Therefore, the detection of EGFR gene mutations is important for determining treatment planning. The aim of the study was to establish a method to detect EGFR gene mutations at codons 746-753. Materials and Methods: EGFR gene mutation at codons 746-753 in six cancer cell lines were investigated. A loop-mediated isothermal amplification (LAMP)-based procedure was developed, that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented LAMP can amplify the DNA fragment of the EGFR gene with codons 746-753 mutations within 30 min. Moreover, boiled cells can work as template resources. Conclusion: Mutation oriented-LAMP assay for EGFR gene mutation is sensitive on extracted DNA. This procedure would be capable of detecting EGFR gene mutation in sputum, pleural effusion, broncho-alveolar lavage fluid or trans-bronchial lung biopsy by chair side.

本文言語English
ページ(範囲)2093-2099
ページ数7
ジャーナルAnticancer research
38
4
DOI
出版ステータスPublished - 2018 4
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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