Detection of avian influenza virus and its antibody by fluorescence polarization immunoassay

Keine Nishiyama, Yohei Takeda, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Haruko Ogawa, Manabu Tokeshi

研究成果: Conference contribution

抄録

This paper reports a high-throughput and rapid method for a fluorescence polarization immunoassay (FPIA) of H5-avian influenza virus (H5-AIV) and anti-H5-AIV antibody using a portable FP analyzer with a microdevice. For both assays, a fluorescein-labeled recombinant fragment of H5-hemagglutinin was prepared. Although the labeled fragment had low molecular-weight, it served as a tracer for the FPIA. Also, a microdevice having multiplex microchannels was designed, allowing simultaneous multiplex analysis at a small sample volume. Consequently, the virus and the antibody in several samples were successfully detected rapidly in the same format.

本文言語English
ホスト出版物のタイトルMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ1226-1227
ページ数2
ISBN(電子版)9781733419017
出版ステータスPublished - 2020
イベント24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
継続期間: 2020 10 42020 10 9

出版物シリーズ

名前MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング
  • 化学 (全般)
  • 制御およびシステム工学

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