Detailed deletion mapping of chromosome band 14q32 in human neuroblastoma defines a 1.1-Mb region of common allelic loss

M. Hoshi, N. Otagiri, H. O. Shiwaku, S. Asakawa, N. Shimizu, Y. Kaneko, R. Ohi, Y. Hayashi, A. Horii

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Neuroblastoma (NB) is a well-known malignant disease in infants, but its molecular mechanisms have not yet been fully elucidated. To investigate the genetic contribution of abnormalities on the long arm of chromosome 14 (14q) in NB, we analysed loss of heterozygosity (LOH) in 54 primary NB samples using 12 microsatellite markers on 14q32. Seventeen (31%) of 54 tumours showed LOH at one or more of the markers analysed, and the smallest common region of allelic loss was identified between D14S62 and D14S987. This region was estimated to be 1-cM long from the linkage map. Fluorescence in situ hybridization also confirmed the loss. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN and ploidy. We further constructed a contig spanning the lost region using bacterial artificial chromosome and estimated this region to be approximately 1.1-Mb by pulsed-field gel electrophoresis. Our results will contribute to cloning and characterizing the putative tumour-associated gene(s) in 14q32 in NB. (C) 2000 Cancer Research Campaign.

本文言語English
ページ(範囲)1801-1807
ページ数7
ジャーナルBritish Journal of Cancer
82
11
DOI
出版ステータスPublished - 2000
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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