TY - JOUR
T1 - Detailed deletion mapping of chromosome band 14q32 in human neuroblastoma defines a 1.1-Mb region of common allelic loss
AU - Hoshi, M.
AU - Otagiri, N.
AU - Shiwaku, H. O.
AU - Asakawa, S.
AU - Shimizu, N.
AU - Kaneko, Y.
AU - Ohi, R.
AU - Hayashi, Y.
AU - Horii, A.
N1 - Funding Information:
The authors are grateful to Dr Tasuke Konno (Tohoku University) for providing samples, and to Dr Barbara Lee Smith Pierce (the Life Science Coordinator for the University of Maryland Asian Division) for editorial work in the preparation of this manuscript. This work was supported by the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000
Y1 - 2000
N2 - Neuroblastoma (NB) is a well-known malignant disease in infants, but its molecular mechanisms have not yet been fully elucidated. To investigate the genetic contribution of abnormalities on the long arm of chromosome 14 (14q) in NB, we analysed loss of heterozygosity (LOH) in 54 primary NB samples using 12 microsatellite markers on 14q32. Seventeen (31%) of 54 tumours showed LOH at one or more of the markers analysed, and the smallest common region of allelic loss was identified between D14S62 and D14S987. This region was estimated to be 1-cM long from the linkage map. Fluorescence in situ hybridization also confirmed the loss. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN and ploidy. We further constructed a contig spanning the lost region using bacterial artificial chromosome and estimated this region to be approximately 1.1-Mb by pulsed-field gel electrophoresis. Our results will contribute to cloning and characterizing the putative tumour-associated gene(s) in 14q32 in NB. (C) 2000 Cancer Research Campaign.
AB - Neuroblastoma (NB) is a well-known malignant disease in infants, but its molecular mechanisms have not yet been fully elucidated. To investigate the genetic contribution of abnormalities on the long arm of chromosome 14 (14q) in NB, we analysed loss of heterozygosity (LOH) in 54 primary NB samples using 12 microsatellite markers on 14q32. Seventeen (31%) of 54 tumours showed LOH at one or more of the markers analysed, and the smallest common region of allelic loss was identified between D14S62 and D14S987. This region was estimated to be 1-cM long from the linkage map. Fluorescence in situ hybridization also confirmed the loss. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN and ploidy. We further constructed a contig spanning the lost region using bacterial artificial chromosome and estimated this region to be approximately 1.1-Mb by pulsed-field gel electrophoresis. Our results will contribute to cloning and characterizing the putative tumour-associated gene(s) in 14q32 in NB. (C) 2000 Cancer Research Campaign.
UR - http://www.scopus.com/inward/record.url?scp=0034025636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034025636&partnerID=8YFLogxK
U2 - 10.1054/bjoc.2000.1108
DO - 10.1054/bjoc.2000.1108
M3 - Article
C2 - 10839294
AN - SCOPUS:0034025636
VL - 82
SP - 1801
EP - 1807
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 11
ER -
