TY - JOUR
T1 - Design of Pyrene-Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release
AU - Hayashi, Keita
AU - Mitsuyoshi, Yuma
AU - Kamei, Toshiyuki
AU - Shimanouchi, Toshinori
AU - Suga, Keishi
AU - Okamoto, Yukihiro
AU - Nakamura, Hidemi
AU - Umakoshi, Hiroshi
N1 - Funding Information:
This work was supported by Grant-in-Aids for Scientific Research (A) (26249116) and Young Scientists (B) (15K18279) from the Japan Society for the Promotion of Science (JSPS).
Funding Information:
This work was supported by Grant-in-Aids for Scientific Research (A) (26249116) and Young Scientists (B) (15K18279) from the Japan Society for the Promotion of Science (JSPS). Murine J774.1 macrophage-like cells and murine cells derived from rectal cancer (Colon 26 cells) were provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/3/31
Y1 - 2018/3/31
N2 - Fluorescence probes are usually employed to analyze pharmacokinetics of drug carriers; however, this method using usual probes is not suitable to monitor drug carriers in detail because fluorescence spectra do not change by the disruption of drug carriers. In this study, pyrene-fatty acid conjugates were investigated as probes to monitor the state of drug carriers in real time. 1-Pyrenemethanol was conjugated with fatty acids, such as lauric acid, stearic acid, and behenic acid, and the conjugates were stirred in ethanol, resulting in the formation of submicron particles; these particles exhibited excimer emission. When J774.1 and Colon 26 cells were treated with these particles, the associated fluorescence spectra shifted from excimer emission to monomer emission. Moreover, the degree of change was controlled by the type of fatty acid. These results support the design of drug carriers that can be used to monitor pharmacokinetics in real time and to control the disruption time.
AB - Fluorescence probes are usually employed to analyze pharmacokinetics of drug carriers; however, this method using usual probes is not suitable to monitor drug carriers in detail because fluorescence spectra do not change by the disruption of drug carriers. In this study, pyrene-fatty acid conjugates were investigated as probes to monitor the state of drug carriers in real time. 1-Pyrenemethanol was conjugated with fatty acids, such as lauric acid, stearic acid, and behenic acid, and the conjugates were stirred in ethanol, resulting in the formation of submicron particles; these particles exhibited excimer emission. When J774.1 and Colon 26 cells were treated with these particles, the associated fluorescence spectra shifted from excimer emission to monomer emission. Moreover, the degree of change was controlled by the type of fatty acid. These results support the design of drug carriers that can be used to monitor pharmacokinetics in real time and to control the disruption time.
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U2 - 10.1021/acsomega.7b02061
DO - 10.1021/acsomega.7b02061
M3 - Article
AN - SCOPUS:85044836851
SN - 2470-1343
VL - 3
SP - 3572
EP - 3580
JO - ACS Omega
JF - ACS Omega
IS - 3
ER -