TY - JOUR
T1 - Design of an LCST-UCST-Like Thermoresponsive Zwitterionic Copolymer
AU - Morimoto, Nobuyuki
AU - Yamamoto, Masaya
N1 - Funding Information:
The authors thank Prof. Hitoshi Takamura (Tohoku University) for his kind help with DLS measurements. M.Y. acknowledges financial support from Japan-Korea Basic Scientific Cooperation Program between the Japan Society for the Promotion of Science (JSPS) and National Research Foundation of Korea (NRF) (JPJSBP120208802). N.M. acknowledges financial support from JSPS (JP17H02096 and JP18H03542).
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/3/23
Y1 - 2021/3/23
N2 - Thermoresponsive polymers that possess both UCST- and LCST-like behaviors have generally been designed using diblock copolymers that are mostly composed of an LCST-like polymer and a UCST polymer. Herein, we prepared an LCST-UCST-type polymer composed of UCST-like thermoresponsive zwitterionic sulfabetaine methacrylate and nonthermoresponsive PEG methacrylate, ZB-PEG, by reversible addition-fragmentation chain transfer (RAFT) copolymerization. By adjusting the PEG composition, ZB-PEG formed a mesoglobule, a microglobule, and the dissociated states in phosphate-buffered saline (PBS). These states were found to be reversible via temperature control. Moreover, this behavior showed high reversibility and succeeded in stabilizing horseradish peroxidase (HRP) in the dilute condition. Such thermoresponsive ZB-PEG can be applied over a wide range of applications in biotechnology and other fields.
AB - Thermoresponsive polymers that possess both UCST- and LCST-like behaviors have generally been designed using diblock copolymers that are mostly composed of an LCST-like polymer and a UCST polymer. Herein, we prepared an LCST-UCST-type polymer composed of UCST-like thermoresponsive zwitterionic sulfabetaine methacrylate and nonthermoresponsive PEG methacrylate, ZB-PEG, by reversible addition-fragmentation chain transfer (RAFT) copolymerization. By adjusting the PEG composition, ZB-PEG formed a mesoglobule, a microglobule, and the dissociated states in phosphate-buffered saline (PBS). These states were found to be reversible via temperature control. Moreover, this behavior showed high reversibility and succeeded in stabilizing horseradish peroxidase (HRP) in the dilute condition. Such thermoresponsive ZB-PEG can be applied over a wide range of applications in biotechnology and other fields.
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U2 - 10.1021/acs.langmuir.0c03128
DO - 10.1021/acs.langmuir.0c03128
M3 - Article
C2 - 33689378
AN - SCOPUS:85103442484
VL - 37
SP - 3261
EP - 3269
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 11
ER -