Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2

K. Iohara, M. Nakashima, M. Ito, M. Ishikawa, A. Nakasima, A. Akamine

研究成果: Article査読

277 被引用数 (Scopus)

抄録

Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation. Abbreviations: BMP2, bone morphogenetic protein 2; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix protein 1; ALPase, alkaline phosphatase; MMP20, matrix metalloproteinase 20; Phex, phosphate-regulating gene with homologies to endopeptidases on X-chromosome.

本文言語English
ページ(範囲)590-595
ページ数6
ジャーナルJournal of dental research
83
8
DOI
出版ステータスPublished - 2004 8
外部発表はい

ASJC Scopus subject areas

  • Dentistry(all)

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