Denopamine stimulates alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator in rat lungs

Xiu Gu, Zheng Wang, Jin Xu, Sumiko Maeda, Makoto Sugita, Motoyasu Sagawa, Hirohisa Toga, Tsutomu Sakuma

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    Objective: The objective of this study was to test the hypothesis that cystic fibrosis transmembrane conductance regulator (CFTR) plays a role in β1-adrenergic agonist-stimulated alveolar fluid clearance. Methods: Isotonic 5% albumin solutions containing different pharmacological agents were instilled into the alveolar spaces of the isolated rat lungs. The lungs were inflated with 100% oxygen at an airway pressure of 7 cm H 2O and placed in a humidified incubator at 37°C. Alveolar fluid clearance was estimated by the progressive increase in the albumin concentration over 1 h. To test the hypothesis, we determined whether CFTR Cl- channel inhibitors (glibenclamide and CFTRinh-172) inhibited the effect of denopamine, a β1-adrenergic agonist, on stimulation of alveolar fluid clearance in the isolated rat lungs. Results: Denopamine increased alveolar fluid clearance in a dose-dependent manner. Atenolol, a β1-adrenergic antagonist, abolished the effects of denopamine on stimulation of alveolar fluid clearance. Although glibenclamide alone or CFTRinh-172 alone did not change basal alveolar fluid clearance, these CFTR inhibitors inhibited the effect of denopamine on alveolar fluid clearance. Conclusion: CFTR plays a role in β1-adrenergic agonist-stimulated alveolar fluid clearance in rat lungs.

    本文言語English
    ページ(範囲)566-571
    ページ数6
    ジャーナルRespirology
    11
    5
    DOI
    出版ステータスPublished - 2006 9 1

    ASJC Scopus subject areas

    • Pulmonary and Respiratory Medicine

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