Delimitation of cohesive ends site (cos) of Streptomyces temperate bacteriophage R4

Hisayuki Mitsui, Hideo Takahashi

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 by from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.

本文言語English
ページ(範囲)1663-1668
ページ数6
ジャーナルBiochemical and biophysical research communications
186
3
DOI
出版ステータスPublished - 1992 8 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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