Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae

Kozo Tsuchiya, Setsuzo Tada, Katsuya Gomi, Katsuhiko Kitamoto, Chieko Kumagai, Gakuzo Tamura

研究成果: Article査読

54 被引用数 (Scopus)

抄録

The Taka-amylase A gene (amy B) of Aspergillus oryzae is induced by starch or maltose. The molecular mechanism of the induction was investigated using a fusion of the amy B promoter and the Escherichia coli uid A gene encoding β-glucuronidase (GUS). To identify the region responsible for high-level expression and regulation within the amy B promoter, a series of deletion promoters was constructed and introduced into the A. oryzae met locus by homologous recombination. Deletion of the region between -377 to -290 (the number indicates the distance in base pairs from the translation initiation point (+1) to the deletion end point) significantly reduced of the GUS activity, but slight reduction of the GUS activity was observed in deletions up to -377. Northern blot analysis showed that reduction of the GUS activity depended upon the expression level of the GUS gene. The region between -377 to -290 is suggested to include the sequence required directly for high-level expression and regulation of the amy B gene.

本文言語English
ページ(範囲)1849-1853
ページ数5
ジャーナルBioscience, Biotechnology and Biochemistry
56
11
DOI
出版ステータスPublished - 1992 1 1
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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