Deformation of lipid droplets in fixed samples

Satoshi Fukumoto, Toyoshi Fujimoto

研究成果: Article査読

132 被引用数 (Scopus)

抄録

Nile red, Sudan III, and oil red O have been used to stain lipid droplets (LDs) for fluorescence microscopy. We noticed that LDs labeled by Nile red are different in appearance from those stained by the latter two dyes. To understand the cause of the difference, we used sequential labeling procedures (first LD stain-photography-quenching-second LD stain-photography), and examined the effect of several factors. Immunofluorescence labeling for adipose differentiation-related protein (ADRP), an LD marker, was also observed comparatively with the lipid stains. As a result, we found that ethanol and isopropanol used for Sudan III and oil red O staining, respectively, and glycerol used for mounting, cause fusion of adjacent LDs even in glutaraldehydefixed samples. By the same treatment, immunofluorescence labeling for ADRP was dislocated to the rim of large LDs that were formed as a result of the artifactual fusion. The result indicates that the LD structure can be better observed with Nile red than with Sudan III or oil red O.

本文言語English
ページ(範囲)423-428
ページ数6
ジャーナルHistochemistry and Cell Biology
118
5
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学

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