Decreased calcium channel currents and facilitated epinephrine release in the Ca2+ channel β3 subunit-null mice

Toshio Ohta, Takayoshi Ohba, Takashi Suzuki, Hiroyuki Watanabe, Hironobu Sasano, Manabu Murakami

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The β subunits of voltage-dependent calcium channels are known to modify calcium channel currents through pore-forming α1 subunits. The β3 subunit is expressed in the adrenal gland and participates in forming various calcium channel types. We performed a series of experiments in β3-null mice to determine the role of the β3 subunit in catecholamine release from the adrenal chromaffin system. Protein levels of N-type channel forming CaV2.2 and L-type forming CaV1.2 decreased. The β3-null mice showed a decreased baroreflex, suggesting decreased sympathetic tonus, whereas plasma catecholamine levels did not change. Pulse-voltage stimulation revealed significantly increased amperometrical currents in β3-null mice, while patch-clamp recordings showed a significant reduction in Ca2+-currents due to reduced L- and N-type currents, indicating facilitated exocytosis. A biochemical analysis revealed increased InsP3 production. In conclusion, our results indicate the importance of the β3 subunit in determining calcium channel characteristics and catecholamine release in adrenal chromaffin cells.

本文言語English
ページ(範囲)464-469
ページ数6
ジャーナルBiochemical and biophysical research communications
394
3
DOI
出版ステータスPublished - 2010 4 9

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Decreased calcium channel currents and facilitated epinephrine release in the Ca<sup>2+</sup> channel β3 subunit-null mice」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル