Cutting edge: Gln22 of mouse MD-2 is essential for species-specific lipopolysaccharide mimetic action of Taxol1

K. Kawasaki, K. Gomi, M. Nishijima

    研究成果: Article査読

    60 被引用数 (Scopus)

    抄録

    MD-2 associates with the extracellular domain of Toll-like receptor 4 (TLR4) and greatly enhances LPS signaling via TLR4. Taxol, which mimics the action of LPS on murine macrophages, induces signals via mouse TLR4-MD-2, but not via human TLR4-MD-2. Here we investigated the molecular basis for this species-specific action of Taxol. Expression of mouse MD-2 conferred both LPS and Taxol responsiveness on human embryonic kidney 293 cells expressing mouse TLR4, whereas expression of human MD-2 conferred LPS responsiveness alone, suggesting that MD-2 is responsible for the species-specificity as to Taxol responsiveness. Furthermore, mouse MD-2 mutants, in which Gln22 was changed to other amino acids, showed dramatically reduced ability to confer Taxol responsiveness, although their ability to confer LPS responsiveness was not affected. These results indicated that Gln22 of mouse MD-2 is essential for Taxol signaling but not for LPS signaling.

    本文言語English
    ページ(範囲)11-14
    ページ数4
    ジャーナルJournal of Immunology
    166
    1
    DOI
    出版ステータスPublished - 2001 1 1

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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