Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal

Hisatoshi Sugiura, Xiangde Liu, Fenghai Duan, Shin Kawasaki, Shinsaku Togo, Koichiro Kamio, Qi Wang Xing, Lijun Mao, Youngsoo Ahn, Ronald F. Ertl, Tom W. Bargar, Abdo Berro, Thomas B. Casale, Stephen I. Rennard

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Asthmatic airway remodeling is characterized by goblet cell hyperplasia, angiogenesis, smooth muscle hypertrophy, and subepithelial fibrosis. This study evaluated whether acquired changes in fibroblast phenotype could contribute to this remodeling. Airway and parenchymal fibroblasts from control or chronically ovalbumin (OVA)-sensitized and challenged "asthmatic" mice were assessed for several functions related to repair and remodeling ± exogenous transforming growth factor (TGF)-β. All OVA-challenged mouse fibroblasts demonstrated augmented gel contraction (P < 0.05) and Chemotaxis (P < 0.05); increased TGF-β1 (P < 0.05), fibronectin (P < 0.05), and vascular endothelial growth factor (P < 0.05) release; and expressed more α-smooth muscle actin (P < 0.05). TGF- aβ1 stimulated both control and asthmatic fibroblasts, which retained all differences from control fibroblasts for all features(P < 0.05, all comparisons). Parenchymal fibroblasts proliferated more rapidly (P < 0.05), while airway fibroblasts proliferated similarly compared with control fibroblasts (P = 0.25). Thus, in this animal model, OVA-challenged mouse fibroblasts acquire a distinct phenotype that differs from control fibroblasts. The augmented profibrotic activity and mediator release of asthmatic fibroblasts could contribute to airway remodeling in asthma.

本文言語English
ページ(範囲)424-430
ページ数7
ジャーナルAmerican journal of respiratory cell and molecular biology
37
4
DOI
出版ステータスPublished - 2007 10
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 呼吸器内科
  • 臨床生化学
  • 細胞生物学

フィンガープリント

「Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル