TY - JOUR
T1 - Crystallization of high-quality protein crystals using an external electric field
AU - Koizumi, Haruhiko
AU - Uda, S.
AU - Fujiwara, K.
AU - Tachibana, M.
AU - Kojima, K.
AU - Nozawa, J.
N1 - Publisher Copyright:
© 2015 International Union of Crystallography.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - The effect of a 20kHz external electric field on the quality of tetragonal hen egg white (HEW) lysozyme crystals was investigated using X-ray diffraction rocking-curve measurements. The full width at half-maximum was found to be larger for high-order reflections but smaller for low-order reflections. In particular, it was revealed that a large amount of local strain is accumulated in tetragonal HEW lysozyme crystals grown under an applied field at 20kHz. Comparison with previous results obtained for crystals grown with an applied field at 1MHz [Koizumi, Uda, Fujiwara, Tachibana, Kojima & Nozawa (2013). J. Appl. Cryst. 46, 25-29] indicated that improvement of the protein crystal quality could be achieved by selection of an appropriate frequency for the applied electric field, which has a significant effect on the growth of the solid.
AB - The effect of a 20kHz external electric field on the quality of tetragonal hen egg white (HEW) lysozyme crystals was investigated using X-ray diffraction rocking-curve measurements. The full width at half-maximum was found to be larger for high-order reflections but smaller for low-order reflections. In particular, it was revealed that a large amount of local strain is accumulated in tetragonal HEW lysozyme crystals grown under an applied field at 20kHz. Comparison with previous results obtained for crystals grown with an applied field at 1MHz [Koizumi, Uda, Fujiwara, Tachibana, Kojima & Nozawa (2013). J. Appl. Cryst. 46, 25-29] indicated that improvement of the protein crystal quality could be achieved by selection of an appropriate frequency for the applied electric field, which has a significant effect on the growth of the solid.
KW - external electric fields
KW - protein crystallization
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U2 - 10.1107/S1600576715015885
DO - 10.1107/S1600576715015885
M3 - Article
AN - SCOPUS:84943328425
VL - 48
SP - 1507
EP - 1513
JO - Journal of Applied Crystallography
JF - Journal of Applied Crystallography
SN - 0021-8898
ER -