SoxR, a member of the MerR family of transcriptional activators, functions as a sensor of oxidative stress. The redox states of the 2Fe-2S cluster of SoxR regulate the activity of SoxR. Here, the crystallization and preliminary crystallographic analysis of SoxR and its complex with DNA are reported. Crystals of SoxR were obtained using PEG 10 000 and glycerol as precipitants. The crystals of SoxR belong to space group P62 or P64, with unit-cell parameters a = b = 80.0, c = 88.1 Å. Crystals of the SoxR-DNA complex were obtained using a 20 bp DNA fragment from a condition containing PEG 10 000 and sodium/potassium tartrate. The crystals of the SoxR-DNA complex belong to space group P6122 or P6522, with unit-cell parameters a = b = 53.5, c = 355.6 Å. Diffraction data were collected to a maximum resolution of 3.2 and 2.7 Å for SoxR and the SoxR-DNA complex, respectively.
|ジャーナル||Acta Crystallographica Section F: Structural Biology and Crystallization Communications|
|出版ステータス||Published - 2006 12|
ASJC Scopus subject areas
- Structural Biology
- Condensed Matter Physics