Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation

Zuoqi Gai, Akiyoshi Nakamura, Yoshikazu Tanaka, Nagisa Hirano, Isao Tanaka, Min Yao

研究成果: Article査読

4 被引用数 (Scopus)

抄録

After crystallization of a certain protein-RNA complex, well diffracting crystals were obtained. However, the asymmetric unit of the crystal was too small to locate any components. Mass spectrometry and X-ray crystal structure analysis showed that it was a member of the DING protein family (HPBP). Surprisingly, the structure of HPBP reported previously was also determined accidentally as a contaminant, suggesting that HPBP has a strong tendency to crystallize. Furthermore, DING proteins were reported to relate in disease. These observations suggest that DING has potential for application in a wide range of research fields. To enable further analyses, a system for preparation of HPBP was constructed. As HPBP was expressed in insoluble form in Escherichia coli, it was unfolded chemically and refolded. Finally, a very high yield preparation method was constructed, in which 43 mg of HPBP was obtained from 1 L of culture. Furthermore, to evaluate the validity of refolding, its crystal structure was determined at 1.03 Å resolution. The determined structure was identical to the native structure, in which two disulfide bonds were recovered correctly and a phosphate ion was captured. Based on these results, it was concluded that the refolded HPBP recovers its structure correctly.

本文言語English
ページ(範囲)854-858
ページ数5
ジャーナルJournal of Synchrotron Radiation
20
6
DOI
出版ステータスPublished - 2013 11
外部発表はい

ASJC Scopus subject areas

  • 放射線
  • 核物理学および高エネルギー物理学
  • 器械工学

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