Cryogenic coherent X-ray diffraction imaging of biological samples at SACLA: A correlative approach with cryo-electron and light microscopy

Yuki Takayama, Koji Yonekura

研究成果: Review article査読

13 被引用数 (Scopus)

抄録

Coherent X-ray diffraction imaging at cryogenic temperature (cryo-CXDI) allows the analysis of internal structures of unstained, non-crystalline, whole biological samples in micrometre to sub-micrometre dimensions. Targets include cells and cell organelles. This approach involves preparing frozen-hydrated samples under controlled humidity, transferring the samples to a cryo-stage inside a vacuum chamber of a diffractometer, and then exposing the samples to coherent X-rays. Since 2012, cryo-coherent diffraction imaging (CDI) experiments have been carried out with the X-ray free-electron laser (XFEL) at the SPring-8 Ångstrom Compact free-electron LAser (SACLA) facility in Japan. Complementary use of cryo-electron microscopy and/or light microscopy is highly beneficial for both pre-checking samples and studying the integrity or nature of the sample. This article reports the authors' experience in cryo-XFEL-CDI of biological cells and organelles at SACLA, and describes an attempt towards reliable and higher-resolution reconstructions, including signal enhancement with strong scatterers and Patterson-search phasing.

本文言語English
ページ(範囲)179-189
ページ数11
ジャーナルActa Crystallographica Section A: Foundations and Advances
72
DOI
出版ステータスPublished - 2016
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生化学
  • 材料科学(全般)
  • 凝縮系物理学
  • 物理化学および理論化学
  • 無機化学

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