Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto, Mika Terauchi, Anna Hugo, Tanner Kim, Yasuyuki Araki, Takehiko Wada

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is “covalently” cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

本文言語English
ページ(範囲)10323-10325
ページ数3
ジャーナルChemical Communications
49
87
DOI
出版ステータスPublished - 2013 10 8

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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