Covalent protein labeling based on noncatalytic β-Lactamase and a designed FRET substrate

Shin Mizukami, Shuji Watanabe, Yuichiro Hori, Kazuya Kikuchi

研究成果: Article査読

115 被引用数 (Scopus)

抄録

Techniques for labeling proteins with small molecules have attracted the attention of many life scientists. We have developed a novel protein labeling system that combines a genetically modified, noncatalytic β-lactamase variant and specific mechanism-based fluorescent probes. Rational design of the tag protein and the labeling probes enables highly specific incorporation of the fluorogen. The feasibility of our approach was confirmed by gel electrophoresis, mass spectrometry, fluorescence spectroscopy, and fluorescence microscopic imaging. Labeling techniques that satisfy the dual criteria of specificity and fluorogenicity have rarely been reported. As a consequence, this method could be a broadly useful research tool in the field of life science.

本文言語English
ページ(範囲)5016-5017
ページ数2
ジャーナルJournal of the American Chemical Society
131
14
DOI
出版ステータスPublished - 2009 4 15
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 化学 (全般)
  • 生化学
  • コロイド化学および表面化学

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