Coronary vascular bed perfusion with a polyethylene glycol-modified hemoglobin-encapsulated liposome, Neo red cell, in rats

Kunihiko Nakai, Akira Usuba, Toshio Ohta, Mikinori Kuwabara, Yoshikazu Nakazato, Ryoichi Motoki, Tsuneo A. Takahashi

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Whether hemoglobin (Hb) encapsulated liposomes have vasoconstrictive activity remains controversial. We therefore examined the vascular activity of a liposome Hb, Neo red cell (NRC), in a simple in vitro model of Langendorff perfusion of the rat heart using KrebsHenseleit (KH) solution as the perfusate. In the KH solution, NRC (Hb at 1 mg/ml), however, induced an immediate and abnormal increase in perfusion pressure. Histological examinations revealed that embolisms were the likely cause of this disturbance. Inorganic crystals formed by the mixing of NRC with the perfusate were a possible source of the embolisms. We found that the addition of bovine serum albumin to the perfusate was effective in avoiding embolic events. This protocol was used to compare the vasoconstrictive properties of unmodified bovine Hb and NRC. Unmodified bovine Hb (1 mg/ml) caused an increase in perfusion pressure and O decrease in the duration of bradykinin- induced relaxation. In contrast, NRC (Hb at 1 mg/ml) had no such vasoconstrictive effects. These results provide the first information regarding perfusion of the circulatory vascular bed by NRC and further evidence that the encapsulation of Hb into liposomes is an effective approach to modulate Hb-related vasoconstrictive activity.

本文言語English
ページ(範囲)320-325
ページ数6
ジャーナルArtificial Organs
22
4
DOI
出版ステータスPublished - 1998

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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