TY - JOUR
T1 - Copper electrodes for stable subpicomole detection of carbohydrates in high-performance liquid chromatography
AU - Kano, Kenji
AU - Takagi, Kazuyoshi
AU - Inoue, Kumi
AU - Ikeda, Tokuji
AU - Ueda, Teruhisa
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Scientific Research No. 05671786 for K. K. from the Ministry of Education, Science and Culture of Japan.
PY - 1996/1/15
Y1 - 1996/1/15
N2 - A new pretreatment of copper (Cu) electrodes used for catalytic oxidation of carbohydrates is presented, which involves superficial polishing of the electrochemically-oxidized Cu electrode and then air-oxidation at 100°C for 3 h. This pretreatment allows highly sensitive and stable detection of carbohydrates at 0.45 V (vs. Ag/AgCl/Sat. KCl) in anion-exchange high-performance liquid chromatography. Glucose was detected at concentration levels as low as 0.2 pmole with a dynamic range up to 10,000 pmole over a period of 1 month. The change in the electrode surface state during the pretreatment is discussed from the electrochemical point of view.
AB - A new pretreatment of copper (Cu) electrodes used for catalytic oxidation of carbohydrates is presented, which involves superficial polishing of the electrochemically-oxidized Cu electrode and then air-oxidation at 100°C for 3 h. This pretreatment allows highly sensitive and stable detection of carbohydrates at 0.45 V (vs. Ag/AgCl/Sat. KCl) in anion-exchange high-performance liquid chromatography. Glucose was detected at concentration levels as low as 0.2 pmole with a dynamic range up to 10,000 pmole over a period of 1 month. The change in the electrode surface state during the pretreatment is discussed from the electrochemical point of view.
UR - http://www.scopus.com/inward/record.url?scp=0030071602&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030071602&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(95)00757-1
DO - 10.1016/0021-9673(95)00757-1
M3 - Article
AN - SCOPUS:0030071602
VL - 721
SP - 53
EP - 57
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1
ER -