Control of gene expression in neurons by the use of in vivo electroporation and the tetracycline system

Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito

研究成果: Chapter

5 被引用数 (Scopus)

抄録

In vivo electroporation is a powerful technique to transfect foreign genes into neural stem/progenitor cells in the developing nervous system. Gene function has been analyzed in transfected neural stem/progenitor cells and their descendants, differentiated neurons. An inducible gene expression system working in neurons is desirable to examine gene function specifically in neurons. We have recently shown that gene induction is strictly controlled in postmitotic neurons in mice at both embryonic and postnatal stages using in vivo electroporation and the newest version of the tetracycline (Tet)-On system. Here we describe the method of mouse in vivo electroporation combined with the Tet-On system for precise spatiotemporal control of gene expression.

本文言語English
ホスト出版物のタイトルElectroporation Methods in Neuroscience
出版社Springer New York
ページ187-195
ページ数9
ISBN(電子版)9781493924592
ISBN(印刷版)9781493924585
DOI
出版ステータスPublished - 2015 3月 9

ASJC Scopus subject areas

  • 医学(全般)
  • 神経科学(全般)

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