Contrast transfer for frozen-hydrated specimens II. Amplitude contrast at very low frequencies

Chikashi Toyoshima, Koji Yonekura, Hiroyuki Sasabe

研究成果: Article査読

45 被引用数 (Scopus)

抄録

The contrast transfer properties of frozen-hydrated specimens were examined quantitatively for low spatial frequencies (1/350-1/160 Å-1). Specimens used were tubular crystal of two membrane protiens, the acetylcholine receptor and calcium-ATPase. These specimens were found to behave as weak-phase-weak-amplitude objects. The contribution of amplitude contrast was 5.8% at 120 kV and 4.8% at 200 kV accelerating voltage. Combined with previous results, the level of amplitude contrast showed little dependence on the spatial frequency over the range examined so far (1/350-1/35 Å-1).

本文言語English
ページ(範囲)165-176
ページ数12
ジャーナルUltramicroscopy
48
1-2
DOI
出版ステータスPublished - 1993 1月
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 器械工学

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