Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter

Miyako Udono, Tsukasa Fujiki, Makiko Yamashita, Kazuyuki Katsuki, Masaaki Funata, Yusuke Imada, Kaichi Yoshizaki, Sanetaka Shirahata, Yoshinori Katakura

研究成果: Article査読

抄録

We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.

本文言語English
ページ(範囲)1638-1641
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
72
6
DOI
出版ステータスPublished - 2008

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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