Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae

Setsuzo Tada, Katsuya Gomi, Katsuhiko Kitamoto, Kojiro Takahashi, Gakuzo Tamura, Shodo Hara

研究成果: Article査読

84 被引用数 (Scopus)

抄録

Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The amount of mRNA homologous to the TAA gene was increased when this fungus was grown with starch as a sole carbon source. In order to analyze the induction mechanism, we inserted the Escherichia coli uidA gene encoding β-glucuronidase (GUS) downstream of the TAA promoter and introduced the resultant fusion gene into the A. oryzae genome. Production of a functional GUS protein was induced by starch, but not by glucose. When the effects of various sugars on expression of the fusion gene were examined, the results suggested that the expression of the fusion gene was under control of the TAA gene promoter.

本文言語English
ページ(範囲)301-306
ページ数6
ジャーナルMGG Molecular & General Genetics
229
2
DOI
出版ステータスPublished - 1991 10 1
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル