Construction of a cell surface engineered yeast aims to selectively recover molybdenum, a rare metal

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

The depletion of rare metals is an issue of major concern since rare metals are limited in the abundance but essential for high technology industry. However, the present rare metal recovery technology by chemical methods has high environmental impact, poor selectivity, and is too expensive to be practical. To resolve these problems, this study aimed to create a rare metal recovery system using yeast, and molybdenum was selected as the first target. A molybdenum binding protein, ModE, which was derived from Escherichia coli was selected. A fusion gene was generated by linking partial modE with a secretion signal and a domain of α-agglutinin to display the ModE on the surface of yeast cells. The expression of fusion protein on the cell surface was detected by immunofluorescence labeling. As for the recovery experiment, the engineered yeast cells were incubated in 10 mM of sodium molybdate solution for 2 h, and the recovery of molybdenum ion was measured by ICP-AES. The results of fluorescence micrographs showed that the designed fusion protein was successfully expressed on yeast cell surface. According to the results of ICP-AES, the cell surface engineered yeast adsorbed molybdenum and the cells after 72~84 h incubation gave the best adsorption. Besides, the results suggested that the optimization of each functional domain in the fusion protein was important. The selectivity and the lower limit of recoverable concentration are under investigation, while this study provides a preliminary result of bio-extraction technology using cell surface engineered yeast.

本文言語English
ホスト出版物のタイトル22nd International Biohydrometallurgy Symposium
編集者Sabrina Hedrich, Axel Schippers, Kathrin Rubberdt, Franz Glombitza, Wolfgang Sand, Wolfgang Sand, Mario Vera Veliz, Sabine Willscher
出版社Trans Tech Publications Ltd
ページ421-424
ページ数4
ISBN(印刷版)9783035711806
DOI
出版ステータスPublished - 2017
イベント22nd International Biohydrometallurgy Symposium, 2017 - Freiberg, Germany
継続期間: 2017 9 242017 9 27

出版物シリーズ

名前Solid State Phenomena
262 SSP
ISSN(印刷版)1012-0394
ISSN(電子版)1662-9779

Other

Other22nd International Biohydrometallurgy Symposium, 2017
国/地域Germany
CityFreiberg
Period17/9/2417/9/27

ASJC Scopus subject areas

  • 原子分子物理学および光学
  • 材料科学(全般)
  • 凝縮系物理学

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