TY - JOUR
T1 - Conserved temperature-dependent expression of RNA-binding proteins in cyanobacteria with different temperature optima
AU - Ehira, Shigeki
AU - Hamano, Takashi
AU - Hayashida, Tsunefusa
AU - Kojima, Kouji
AU - Nakamoto, Hitoshi
AU - Hiyama, Tetsuo
AU - Ohmori, Masayuki
AU - Shivaji, Sisinthy
AU - Sato, Naoki
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the JSPS to N.S. (no. 13440234), to H.N. (no. 13640640) and a Collaborative Research Program, Graduate University for Advanced Studies (Extreme Environments).
PY - 2003/8/8
Y1 - 2003/8/8
N2 - The expression of the rbp genes, which encode small RNA-binding proteins with a single RNA-recognition motif, is known to increase at low temperature in Anabaena variabilis M3. The 5′-untranslated region (UTR) of the rbpA1 gene is involved in the cold-regulation. We compared the regulation of the rbp genes in three strains of cyanobacteria having different temperature optima, namely, a mesophilic strain Anabaena sp. PCC 7120, a thermophilic strain Thermosynechococcus elongatus BP-1, and a psychrophilic Antarctic strain Oscillatoria sp. SU1. In Anabaena 7120 and T. elongatus, all the rbp gene sequences are known, and the 5′-UTR sequences of some rbp genes have a high similarity to the 5′-UTR of rbpA1. We found that transcripts as well as protein products of these rbp genes accumulated at low temperature. In addition, the expression of rbp genes increased at low temperature in the Oscillatoria sp. SU1. This suggests that a mechanism of cold-regulation of rbp genes is common among various species of cyanobacteria that belong to different taxa and have different temperature optima.
AB - The expression of the rbp genes, which encode small RNA-binding proteins with a single RNA-recognition motif, is known to increase at low temperature in Anabaena variabilis M3. The 5′-untranslated region (UTR) of the rbpA1 gene is involved in the cold-regulation. We compared the regulation of the rbp genes in three strains of cyanobacteria having different temperature optima, namely, a mesophilic strain Anabaena sp. PCC 7120, a thermophilic strain Thermosynechococcus elongatus BP-1, and a psychrophilic Antarctic strain Oscillatoria sp. SU1. In Anabaena 7120 and T. elongatus, all the rbp gene sequences are known, and the 5′-UTR sequences of some rbp genes have a high similarity to the 5′-UTR of rbpA1. We found that transcripts as well as protein products of these rbp genes accumulated at low temperature. In addition, the expression of rbp genes increased at low temperature in the Oscillatoria sp. SU1. This suggests that a mechanism of cold-regulation of rbp genes is common among various species of cyanobacteria that belong to different taxa and have different temperature optima.
KW - 5′-UTR
KW - Cold-regulation
KW - Cyanobacteria
KW - RNA-binding protein
UR - http://www.scopus.com/inward/record.url?scp=0142011471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142011471&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(03)00503-2
DO - 10.1016/S0378-1097(03)00503-2
M3 - Article
C2 - 12900032
AN - SCOPUS:0142011471
VL - 225
SP - 137
EP - 142
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -