Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi, Erina Yazaki, Kazuhei Kudo, Toshifumi Inada

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

本文言語English
ページ(範囲)3254-3263
ページ数10
ジャーナルFEBS Letters
590
18
DOI
出版ステータスPublished - 2016 9月 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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