Congenic method in the chick limb buds by electroporation

Takayuki Suzuki, Toshihiko Ogura

研究成果: Review article査読

16 被引用数 (Scopus)

抄録

Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble-shooting techniques are described.

本文言語English
ページ(範囲)459-465
ページ数7
ジャーナルDevelopment Growth and Differentiation
50
6
DOI
出版ステータスPublished - 2008 8月

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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