Confinement of fluorescent probes in microwells on Si substrates by sealing with lipid bilayers

Koji Sumitomo, Yukihiro Tamba, Youichi Shinozaki, Keiichi Torimitsu

研究成果: Article査読

26 被引用数 (Scopus)

抄録

We investigated the optimum architecture for confining fluorescent probes in microwells on a Si substrate by covering it with a lipid bilayer. We modified the structure of the wells to prevent the lipid membrane from falling into them, and the overhang shape at the aperture improved the probability of confinement. The fluorescence intensity from the calcein confined in the wells remained unchanged for one hour or more, indicating that the probes remain stably in the wells without flowing out. An artificial cell sealed with the suspended membrane is a promising tool for the functional analysis of membrane proteins.

本文言語English
論文番号107001
ジャーナルApplied Physics Express
3
10
DOI
出版ステータスPublished - 2010 10 1
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

フィンガープリント

「Confinement of fluorescent probes in microwells on Si substrates by sealing with lipid bilayers」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル