Conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles

Enrique Valera, Javier Ramón-Azcón, F. J. Sanchez, M. P. Marco, Ángel Rodríguez

研究成果: Article査読

51 被引用数 (Scopus)

抄録

A novel conductimetric immunosensor for atrazine detection has been designed and developed. This immunosensor is mainly based on antibodies labelled with gold nanoparticles. Additionally, the immunosensor consists of an array of two coplanar non-passivated interdigitated metallic μ-electrodes (IDμE) and immunoreagents specifically developed to detect this pesticide. The chemical recognition layer was covalent immobilized on the interdigital space. Immunochemical detection of the concentration of atrazine is achieved by a competitive reaction that occurs before the inclusion of the labelled antibodies. It is shown that the gold nanoparticles provide an amplification of the conductive signal and hence makes possible to detect atrazine by means of simple DC measurements. The conductimetric immunosensor and its biofunctionalization steps have been characterized by chemical affinity methods and impedance spectroscopy. This work describes the immunosensor structure, fabrication, physico-chemical and analytical characterization, and the immunosensor response using conductivity measurements. The immunosensor developed detects atrazine with limits of detection in the order of 0.1-1 μg L-1, far below the maximum residue level (MRL) (100 μg L-1) established by European Union (EU) for residues of this herbicide in the wine. Although in this paper the competitive reaction occurs in buffer, an initial study of the wine matrix effect is also described.

本文言語English
ページ(範囲)95-103
ページ数9
ジャーナルSensors and Actuators, B: Chemical
134
1
DOI
出版ステータスPublished - 2008 8月 28
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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