Conditional activation of RhoA suppresses the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation

Toshimitsu Fuse, Yoshiakira Kanai, Masami Kanai-Azuma, Misao Suzuki, Kazuhiro Nakamura, Hisashi Mori, Yoshihiro Hayashi, Masayoshi Mishina

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Gastrulation is a pivotal event of mouse early embryogenesis. In telencephalin (TLCN)-Cre mice carrying the Cre recombinase gene inserted into the translational initiation site of the TLCN gene, Cre-mediated recombination took place at the postimplantation stage. To examine the role of RhoA signaling in early embryogenesis, we produced Rho36 mice carrying constitutively active RhoAG14V gene inducible by Cre recombinase and crossed with TLCN-Cre mice. In doubly transgenic embryos at the gastrulation stage, there appeared an abnormal bulge of cells protruded from the primitive streak region into the amniotic cavity. The bulged cell mass expressed the epiblast marker gene Oct3 and E-cadherin, but not the primitive streak marker gene T except for the basal portion. These results suggest that the conditional activation of RhoA signaling suppressed the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation.

本文言語English
ページ(範囲)665-672
ページ数8
ジャーナルBiochemical and biophysical research communications
318
3
DOI
出版ステータスPublished - 2004 6 4

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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