Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice

Keizo Takao, Koichi Tanda, Kenji Nakamura, Jiro Kasahara, Kazuki Nakao, Motoya Katsuki, Kazuo Nakanishi, Nobuyuki Yamasaki, Keiko Toyama, Minami Adachi, Masahiro Umeda, Tsutomu Araki, Kohji Fukunaga, Hisatake Kondo, Hiroyuki Sakagami, Tsuyoshi Miyakawa

研究成果: Article査読

57 被引用数 (Scopus)

抄録

Calcium-calmodulin dependent protein kinase IV (CaMKIV) is a protein kinase that activates the transcription factor CREB, the cyclic AMP-response element binding protein. CREB is a key transcription factor in synaptic plasticity and memory consolidation. To elucidate the behavioral effects of CaMKIV deficiency, we subjected CaMKIV knockout (CaMKIV KO) mice to a battery of behavioral tests. CaMKIV KO had no significant effects on locomotor activity, motor coordination, social interaction, pain sensitivity, prepulse inhibition, attention, or depression-like behavior. Consistent with previous reports, CaMKIV KO mice exhibited impaired retention in a fear conditioning test 28 days after training. In contrast, however, CaMKIV KO mice did not show any testing performance deficits in passive avoidance, one of the most commonly used fear memory paradigms, 28 days after training, suggesting that remote fear memory is intact. CaMKIV KO mice exhibited intact spatial reference memory learning in the Barnes circular maze, and normal spatial working memory in an eight-arm radial maze. CaMKIV KO mice also showed mildly decreased anxiety-like behavior, suggesting that CaMKIV is involved in regulating emotional behavior. These findings indicate that CaMKIV might not be essential for fear memory or spatial memory, although it is possible that the activities of other neural mechanisms or signaling pathways compensate for the CaMKIV deficiency.

本文言語English
論文番号e9460
ジャーナルPloS one
5
3
DOI
出版ステータスPublished - 2010 3月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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