Comprehensive analysis, with an in-house cDNA microarray, of gene expression in the diabetic mouse retina

Nanami Uehara-Adachi, Atsushi Mizota, Emiko Adachi-Usami, Shuichi Yamamoto, Akinori Ishihara, Katsuro Iwase, Satoko Ohtsuka, Masaki Takiguchi, Masaki Kato, Yoshinori Nimura, Naohiko Seki, Hiroki Kodama

研究成果: Article査読

抄録

Purpose: To clarify the molecular pathophysiology of diabetic retinopathy (DR), we performed comprehensive gene expression analysis of the mouse retina under diabetic conditions with an in-house cDNA microarray. Methods: We prepared a microarray harboring 2,880 cDNAs derived from a mouse retina. Amplified cRNAs of diabetic and age-matched control mouse retinas at 1 and 3 months after intraperitoneal injection of streptozotocin were subjected to array analysis. Results: Genes significantly up-regulated in the diabetic state were listed, and they included a number of genes for oxidative phosphorylation in mitochondoria. Conclusions: Our finding of up-regulation of genes for oxidative phosphorylation in the diabetic retina differs from recently reported findings of down-regulation of these genes in the skeletal muscle of diabetic mice. The diabetic retina seems to be in a potentially activated state for glucose metabolism, presumably because of an increase in insulin-independent glucose influx.

本文言語English
ページ(範囲)85-89
ページ数5
ジャーナルFolia Ophthalmologica Japonica
56
2
出版ステータスPublished - 2005 2月
外部発表はい

ASJC Scopus subject areas

  • 眼科学

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