Comprehensive analysis and characterization of the TCR α chain sequences in the common marmoset

Yoshiki Fujii, Takaji Matsutani, Kazutaka Kitaura, Satsuki Suzuki, Tsunetoshi Itoh, Tomohiko Takasaki, Ryuji Suzuki, Ichiro Kurane

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The common marmoset (Callithrix jacchus) is useful as a nonhuman primate model of human diseases. Although the marmoset model has great potential for studying autoimmune diseases and immune responses against pathogens, little information is available regarding the genes involved in adaptive immunity. Here, we identified one TCR α constant (TRAC), 46 TRAJ (joining), and 35 TRAV (variable) segments from marmoset cDNA. Marmoset TRAC, TRAJ, and TRAV shared 80%, 68-100%, and 79-98% identity with their human counterparts at the amino acid level, respectively. The amino acid sequences were less conserved in TRAC than in TCRβ chain constant (TRBC). Comparative analysis of TRAV between marmosets and humans showed that the rates of synonymous substitutions per site (d S ) were not significantly different between the framework regions (FRs) and complementarity determining regions (CDRs), whereas the rates of nonsynonymous substitutions per site (d N ) were significantly lower in the FRs than in CDRs. Interestingly, the d N values of the CDRs were greater for TRBV than TRAV. These results suggested that after the divergence of Catarrhini from Platyrrhini, amino acid substitutions were decreased in the FRs by purifying selection and occurred more frequently in CDRβ than in CDRα by positive selection, probably depending on structural and functional constraints. This study provides not only useful information facilitating the investigation of adaptive immunity using the marmoset model but also new insight into the molecular evolution of the TCR heterodimer in primate species.

本文言語English
ページ(範囲)383-395
ページ数13
ジャーナルImmunogenetics
62
6
DOI
出版ステータスPublished - 2010 6月
外部発表はい

ASJC Scopus subject areas

  • 免疫学
  • 遺伝学

フィンガープリント

「Comprehensive analysis and characterization of the TCR α chain sequences in the common marmoset」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル