TY - JOUR
T1 - Composition and oligosaccharides of a milk sample of the giant panda, Ailuropoda melanoleuca
AU - Nakamura, Tadashi
AU - Urashima, Tadasu
AU - Mizukami, Taiji
AU - Fukushima, Michihiro
AU - Arai, Ikichi
AU - Senshu, Tatsudo
AU - Imazu, Koji
AU - Nakao, Tatsuko
AU - Saito, Tadao
AU - Ye, Zhiyong
AU - Zuo, Hong
AU - Wu, Kongju
N1 - Funding Information:
We thank Dr M. Messer of the University of Sydney, Australia, for his invaluable suggestions and comments. This study was supported by a grants-in-aid for science research (B) from Japan Society for the Promotion Science. This study was partially supported by a grant from The 21st Century COE program (A-1), Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - A milk sample from a captive giant panda (Ailuropoda melanoleuca), obtained at 13 days postpartum, contained 7.1% protein, 1.6% carbohydrate, 10.4% lipid and 0.9% ash. The ratio of casein to whey proteins was 5.0:2.1. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) of the whey protein fraction showed the presence of at least two major proteins other than α-lactalbumin and β-lactoglobulin. SDS-PAGE and urea-gel electrophoresis showed that αs-casein is not a major component. The proportions of triacylglycerol, cholesterol, cholesterol esters and phospholipid were 90.5, 5.3, 0.96 and 3.1%, of the total lipid, respectively. The dominant saccharide in the panda milk was Gal(α1-3)Gal(β1-4)Glc (isoglobotriose). The milk contained, in addition, lesser amounts of lactose, Gal(α1-3)Gal(β1-4)[Fuc(α1-3)]Glc (fucosyl isoglobotriose), Neu5Ac(α2-3)Gal(β1-4)Glc (3′-N-acetylneuraminyl-lactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6′-N-acetylneuraminyl-lactose) and Neu5Ac(α2-3)Gal(β1-4)[Fuc(α1-3)]Glc.
AB - A milk sample from a captive giant panda (Ailuropoda melanoleuca), obtained at 13 days postpartum, contained 7.1% protein, 1.6% carbohydrate, 10.4% lipid and 0.9% ash. The ratio of casein to whey proteins was 5.0:2.1. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) of the whey protein fraction showed the presence of at least two major proteins other than α-lactalbumin and β-lactoglobulin. SDS-PAGE and urea-gel electrophoresis showed that αs-casein is not a major component. The proportions of triacylglycerol, cholesterol, cholesterol esters and phospholipid were 90.5, 5.3, 0.96 and 3.1%, of the total lipid, respectively. The dominant saccharide in the panda milk was Gal(α1-3)Gal(β1-4)Glc (isoglobotriose). The milk contained, in addition, lesser amounts of lactose, Gal(α1-3)Gal(β1-4)[Fuc(α1-3)]Glc (fucosyl isoglobotriose), Neu5Ac(α2-3)Gal(β1-4)Glc (3′-N-acetylneuraminyl-lactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6′-N-acetylneuraminyl-lactose) and Neu5Ac(α2-3)Gal(β1-4)[Fuc(α1-3)]Glc.
KW - Casein
KW - Fatty acid
KW - Giant panda
KW - Lipids
KW - Milk
KW - Oligosaccharides
KW - Structure
KW - Whey proteins
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U2 - 10.1016/S1096-4959(03)00093-9
DO - 10.1016/S1096-4959(03)00093-9
M3 - Article
C2 - 12831764
AN - SCOPUS:10744224132
VL - 135
SP - 439
EP - 448
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 3
ER -