Comparison of separation methods for tissue-derived extracellular vesicles in the liver, heart, and skeletal muscle

Adam Matejovič, Shohei Wakao, Masaaki Kitada, Yoshihiro Kushida, Mari Dezawa

研究成果: Article査読

抄録

Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation-based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV-related markers, with minimum levels of intracellular organelle-related markers. Some tissue-specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.

本文言語English
ページ(範囲)482-493
ページ数12
ジャーナルFEBS Open Bio
11
2
DOI
出版ステータスPublished - 2021 2

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Comparison of separation methods for tissue-derived extracellular vesicles in the liver, heart, and skeletal muscle」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル