Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats

Hidetoshi Mitani, Ichiro Takahashi, Kazuyuki Onodera, Jin Wan Bae, Takuichi Sato, Nobuhiro Takahashi, Yasuyuki Sasano, Kaoru Igarashi, Hideo Mitani

研究成果: Article査読

22 被引用数 (Scopus)

抄録

A disintegrin and metalloproteinase with thrombospondin motif (adamalysin-thrombospondins, ADAMTS) degrades aggrecan, one of the major extracellular matrix (ECM) components in cartilage. Mandibular condylar cartilage differs from primary cartilage, such as articular and growth plate cartilage, in its metabolism of ECM, proliferation, and differentiation. Mandibular condylar cartilage acts as both articular and growth plate cartilage in the growing period, while it remains as articular cartilage after growth. We hypothesized that functional and ECM differences between condylar and primary cartilages give rise to differences in gene expression patterns and levels of aggrecan and ADAMTS-1, -4, and -5 during growth and aging. We employed in situ hybridization and semiquantitative RT-PCR to identify mRNA expression for these molecules in condylar cartilage and primary cartilages during growth and aging. All of the ADAMTSs presented characteristic, age-dependent expression patterns and levels among the cartilages tested in this study. ADAMTS-5 mainly contributed to ECM metabolism in growth plate and condylar cartilage during growth. ADAMTS-1 and ADAMTS-4 may be involved in ECM turn over in articular cartilage. The results of the present study reveal that ECM metabolism and expression of related proteolytic enzymes in primary and secondary cartilages may be differentially regulated during growth and aging.

本文言語English
ページ(範囲)371-380
ページ数10
ジャーナルHistochemistry and Cell Biology
126
3
DOI
出版ステータスPublished - 2006 9

ASJC Scopus subject areas

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学

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